FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_TGA5_Col09Y_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_TGA5_Col09Y_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14226
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGG150.002179803870.000012
TATACGG252.6990892E-670.000012
ACTTGTT150.002179803870.000019
TTACCGG150.002179803870.000012
GGACTTA700.070.000017
GGACTGG150.002179803870.000017
GGACTGA353.3742253E-970.000017
GGACTCA150.002179803870.000017
CCCGGAC401.200533E-1070.000014
GGACTAC309.5333235E-870.000017
TGATCTA150.002179803870.0000164
TCGCGGA207.662494E-570.000013
GACGGAC207.662494E-570.000014
GCGCGGA150.002179803870.000013
ATGCGGA207.662494E-570.000013
ACCCGGA207.662494E-570.000013
GAAGATG150.002179803870.0000115
AACCCGG150.002179803870.000012
ATAACGG150.002179803870.000012
ATTACGG150.002179803870.000012
Produced by FastQC (version 0.11.5)