FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_TGA5_Col05Y_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_TGA5_Col05Y_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211710
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTA252.8329887E-670.000011
GGGCATA301.020253E-770.01
TAGGACG207.9137084E-570.029
TCCCAGC150.00222239170.055
GCCGTAA207.9137084E-570.02
GGGGCTA207.9137084E-570.01
GGCTAAT850.061.7647063
GGGTAAT3350.058.5074653
GGTAATC3150.056.6666684
CGGTAAT1550.056.4516143
GGCGGTA252.3865682E-456.0000041
GCATACG252.3865682E-456.00000464
GTAATCC1100.054.090915
GGGATAA1750.054.02
GTAATCT4400.053.2954565
GGGGCAG200.006944244752.552
GAGTCGC200.006944244752.534
GCGTTCG200.006944244752.536
GGGGTAA1200.052.52
TGGGGCA200.006944244752.551
Produced by FastQC (version 0.11.5)