FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_TGA5_2019poolH_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_TGA5_2019poolH_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452296
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAACG150.00222212170.015151
GCGGCTC353.6998244E-970.015151
GCGGCCG301.02134436E-770.015151
GGGCGCT4250.068.3526153
GCGCTAT4700.067.765635
GCCCGCT2850.067.543543
GTGCCGC1300.067.314822
AGGGCGC1250.067.207112
GGTCCTA13400.067.126554
GGGTCCT12150.067.119023
AGGTCCT5800.066.982443
GCCGCTA3200.066.718444
GTCCTAT14800.066.4523855
GGCCGCT2850.066.3154753
CGGTCCT4550.066.1535343
GGGCCGC1700.065.889332
ACCGCTA2550.065.882044
GAGGTCC2450.065.7212452
GGTGTCC3250.065.699262
AGGGGCG800.065.6392061
Produced by FastQC (version 0.11.5)