FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_LHY1_F1b_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_LHY1_F1b_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046460
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1253.5597623E-927.99963850
ATGCCGT1551.2896635E-924.83838749
GTTCGGG851.3090258E-424.7055637
GTATGCC1851.4551915E-1124.59427647
GCCGTCT1052.0406062E-523.3330351
CGTATGC1905.638867E-1022.10497546
TATGCCG1905.638867E-1022.10497548
CGCCCCT800.002666449921.8757616
CCGCCCC900.00527549219.4451225
TCGTATC3450.019.27511238
AGTCACG3950.018.60735328
GAGCCGG950.007210722718.42081363
GTCACGA4300.017.90674429
CGTATCT3400.017.49977339
CCGTCTT1807.150662E-515.55535452
CAGTCAC5000.015.399827
ATTCGTA4800.015.31230236
CATCAGG1951.4143117E-414.358788536
TTCGTAT5550.013.87369437
TCCAGTC5850.013.76116325
Produced by FastQC (version 0.11.5)