FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_LHY1_F1a_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_LHY1_F1a_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2226003
Sequences flagged as poor quality0
Sequence length76
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4200.039.16596648
GTATGCC4600.036.52108447
CGTATGC4400.034.2039346
GTCACGA9450.033.33273329
TGCCGTC3700.033.10751350
AGTCACG9700.032.11282728
ATGCCGT4200.031.666149
CAGTCAC11200.028.12449527
TCGTATC8850.027.28825838
CACGAAT11500.027.0864731
CCAGTCA12050.026.4310626
GCCGTCT3750.026.13286451
TCCAGTC12500.025.75953725
CTCCAGT12900.024.6894824
ACTCCAG13450.023.15943523
CGAATTC13300.022.63168133
TCGTATG7300.022.05439845
TCACGAA14650.021.74022330
CTCGTAT7250.021.7237544
ACGAATT14500.021.24147432
Produced by FastQC (version 0.11.5)