FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_LHY1_Col_UB_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_LHY1_Col_UB_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3330575
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAAT95300.065.411743
CGGTAAT50100.063.9944733
GGGCGTA9600.063.445651
GGTAATC75300.063.215294
GGATAAT114000.063.0945243
GCGTAAT39200.062.859563
GGGGTAA36000.062.324642
GGCTAAT51150.062.20183
AGGTAAT93600.062.1872143
GTAATCT99950.061.4221465
GAGTAAT116550.061.383743
GCGGTAA23100.061.3687552
GGTAATA133650.061.0451974
GGTAATT92750.060.982564
GGGAGTA26750.060.7180821
AGTAATC85000.060.6543584
GTAATAT175800.059.9871835
GTAATAG77200.059.9366345
GGGCTAA21650.059.8202362
GATAATC83750.059.637224
Produced by FastQC (version 0.11.5)