FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_LHY1_Col_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_LHY1_Col_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393997
Sequences flagged as poor quality0
Sequence length76
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAT20900.064.47973
GGGTAAT41200.063.9744153
GGGCGTA3950.063.8081
GGTAATC26450.062.4632954
GAGTAAT46850.062.3110433
GCGTAAT14000.062.0057833
GGATAAT54000.061.903923
GGGAGTA11000.061.4192281
GTAATAG31950.061.242025
GGCTAAT21000.061.1723753
GGTAATA67100.061.1383444
GGGGTAA16000.060.820352
GCGGTAA9200.060.496942
GGAGTAA21050.060.3640862
AGGTAAT37000.060.3569833
GTAATAT98150.060.2705235
AGTAATC31300.060.0535244
GGCGGTA5200.059.913731
GGTAATG19800.059.7530484
GGGATAA21850.059.5957952
Produced by FastQC (version 0.11.5)