FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_LHY1_Col16cycle_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_LHY1_Col16cycle_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6820310
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6650.020.52610650
TATGCCG7800.017.94853448
ATGCCGT7650.017.38544349
GCCGTCT7500.017.26648951
CGTATGC9350.016.0960946
GTATGCC8650.015.78018547
CCGTCTT14750.09.01685752
CTCGTAT14800.08.51342644
CAGTCAC17700.08.30499927
TCGTATG16200.08.20979245
CTGCTTG16800.07.7083159
CGGAGTG4750.00342180047.368345748
GATCTCG14655.456968E-126.928256541
TGGGGGG6107.184209E-46.8855795
TCCAGTC22550.06.67408725
CCAGTCA21150.06.619414326
CGTCTTC20700.06.594135353
CGATCTC18150.06.363570740
GAACTCC32900.06.063812370
ACGTCTG39600.05.921699569
Produced by FastQC (version 0.11.5)