FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_LHY1_C24_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_LHY1_C24_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1341393
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCG15780.1176389022456506TruSeq Adapter, Index 7 (97% over 35bp)
GGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCG14910.11115310725492082TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTC301.024091E-770.003285
GAGGCGC17650.069.810172
GGGCGCT70100.069.803553
GGCGCTA98150.069.7536544
GGGGCCG11400.069.704051
AGGGCGC19050.069.6412
GCGCTAT98350.069.576225
CGCGCTA27150.069.48764
AGGCGCT38350.069.4556663
GCGGCGC12250.069.4370042
CGCGCTG5950.069.4150164
GCGCTAA61200.069.316985
GGCGCTC35500.069.313114
GACGCTA38500.069.275984
GCGCTCA32750.069.255155
CGGGCGC13700.069.241992
CGCTATC45450.069.235756
TGGGCGC13550.069.2335052
CGCTATA122050.069.231556
CGGCGCT30600.069.202593
Produced by FastQC (version 0.11.5)