FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_Col_input_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_Col_input_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57413167
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG70300.021.45799448
TGCCGTC66550.019.30115550
CGTATGC79450.018.3259746
ATGCCGT78700.017.61111649
GTATGCC89900.017.20797347
GCCGTCT62950.016.95770651
TCGTATC182900.012.30443938
GTCACGA220500.011.2697329
AGTCACG233500.010.80717428
CTCGTAT136300.010.70798544
CGTATCT172250.010.68784839
CACGAAT242950.010.47326431
TCGTATG145400.09.72488645
TATCTCG170000.09.69696341
ATTCGTA281500.09.23794436
GAATTCG281100.09.20128534
CGAATTC285450.09.09785733
CCGTCTT138400.08.87634252
CAGTCAC294700.08.61038927
TCTCGTA178850.08.37568843
Produced by FastQC (version 0.11.5)