FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_ColSPL9_F1b_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_ColSPL9_F1b_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208987
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1500.032.66651550
GCCGTCT1750.027.99986651
ATGCCGT2050.027.31694249
TATGCCG2250.026.4443248
GTATGCC3150.023.33322347
CGTATGC3100.022.58053846
CATTCAG7900.018.60750833
AGCGTCA950.007211011418.42096540
CCGTCTT3101.8189894E-1218.0644352
GCAGGCT1401.8658454E-417.49991830
CAGTCAC9000.015.94436927
TCGTATG3803.6379788E-1215.6578245
AGCTACG1350.002946004715.5561254
CGGTAAG1350.00294681715.55548154
CCAGTCA9500.015.10519226
TACCGCG1400.00375418914.99992931
TCCAGTC9900.014.49488225
TACGGAG1450.004738939514.4832895
GTCACAT9750.014.35890629
CTCGTAT4252.7284841E-1113.99993444
Produced by FastQC (version 0.11.5)