FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_ColSPL9_F1a_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_ColSPL9_F1a_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336312
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT7170.21319489045885961TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC900.054.44375250
GGCCGGA200.00694813252.49933229
GCGTATG200.00694813252.49933245
CTGGCGC200.00694813252.49933250
GCCGTCT800.048.1243951
GTATGCC1350.046.66607347
ATGCCGT1150.045.6515949
TATGCCG1250.041.99946648
CATTCAG2950.035.59276633
CGTATGC1800.034.99955746
CCGTCTT1102.3646862E-1134.99955452
CTCGTAT1350.033.70327844
CAGTCAC3100.032.74151627
CCAGTCA3100.032.74151626
TCCAGTC3200.031.71834825
TAGGCCT450.00430443931.1107169
TCGTATG1750.029.99961745
ACTCCAG3450.029.41991423
GTCACAT3600.029.16629629
ATCGCGT500.007203679527.99964342
Produced by FastQC (version 0.11.5)