FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_ColSPL9_Col_UB_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_ColSPL9_Col_UB_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306481
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTA252.8308405E-670.0215451
CGGGATA550.070.0215451
GGCGCTA252.8308405E-670.0215451
ACGCGTA207.9072925E-570.0215451
CGTAGTG207.920088E-569.99869570
GGGCCCT150.002223507569.99869541
CGGCGTG1200.067.08208570
GCGGTAA1650.065.777822
GGGCGTA700.065.0200041
CGTCGTG1800.064.16547470
GGTAATC4100.064.0336464
GGGTAAT6150.063.7490543
CGGTAAT4000.063.0091063
GGGCTAA2400.062.7276342
GGGATAA4150.062.428852
GGTAATT7950.061.644134
AGGTAAT7500.061.6089063
GCCCTAA401.0611984E-861.2688522
GCGTAAT3450.060.8783653
GGTTAAT8400.060.8421253
Produced by FastQC (version 0.11.5)