FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_ColSPL9_Col_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_ColSPL9_Col_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201893
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTA150.002214046270.0652161
AGCGTAA353.6652636E-970.065212
ACCTACT150.002222770869.9957929
GGGGTTA800.065.6861341
GCGTAAT1550.065.512393
GGGGCTA700.065.0605551
TAATGCC650.064.6435246
GGGGATA1150.063.9725881
GGCTAAT3150.063.3609163
GGGTAAT5750.063.3319133
GAGTAAT6050.063.0853083
CGGGGTA501.4551915E-1163.058691
CGGGTAA950.062.6899262
GGATAAT6500.062.488743
GCGGTAA1350.062.280192
GTAATAC2700.062.2493175
GGGATAA2550.061.8222472
GGTAATG3300.061.5419434
GTAATAG4000.061.276675
GTAATCT4750.061.184535
Produced by FastQC (version 0.11.5)