FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_ColSPL9_C24_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_ColSPL9_C24_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785628
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA41020.5221300666473191TruSeq Adapter, Index 13 (97% over 38bp)
GGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA36760.4679059300330436TruSeq Adapter, Index 13 (97% over 38bp)
CGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA13930.1773103809945674TruSeq Adapter, Index 13 (97% over 38bp)
TGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA12160.15478063409145296TruSeq Adapter, Index 13 (97% over 38bp)
CGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA11560.14714343175141414TruSeq Adapter, Index 13 (97% over 38bp)
TGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA11180.14230653693605627TruSeq Adapter, Index 13 (97% over 38bp)
GGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA9930.1263956987276421TruSeq Adapter, Index 13 (97% over 38bp)
GGCGACGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA8760.11150315416456644TruSeq Adapter, Index 13 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGCCG7050.070.004391
ACTGCGC1150.069.999932
GGCGCTA55450.069.4318544
CCGCTAG42300.069.4207465
GGTCGCT17600.069.204483
GCCGCTA51150.069.1788254
CGCTAGA86000.069.145296
CGCTATA40350.069.132526
GCGCTAT41650.069.075575
GCGCTCA22450.069.064525
GCGCTAG41150.069.064345
CCGCTAT37350.069.062865
CGCTATT46350.069.018276
CGCTAGT20950.068.997556
CCCGCTA16850.068.9613654
GCCGCTC12950.068.918854
GGGCCGC12650.068.893222
TCGCTAG24650.068.864045
GTCGCTA30800.068.863584
GGGGACG2950.068.817871
Produced by FastQC (version 0.11.5)