FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_ColCAMTA1_F1b_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_ColCAMTA1_F1b_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273801
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAAT37050.065.278813
GCGTAAT18400.065.05653
GGGGTAA14350.064.641362
GGGGATA9250.063.1987231
GGGGCTA4850.062.7929731
CGGTAAT18550.062.4548953
GGTAATC29250.062.343954
GGATAAT46700.062.207623
GAGTAAT43250.061.8286323
GGGCTAA9900.061.522022
GTAATCT37150.061.3344655
AGTAATC33550.061.1346634
GGGATAA18950.060.956672
GGTAATG18100.060.9136164
AGGTAAT35150.060.5425453
GTAATAG32450.060.5104755
GCCTAAT12800.060.4316863
GGCTAAT21900.060.4129563
GTAATAT71000.060.2907265
GGCGGTA4850.059.905941
Produced by FastQC (version 0.11.5)