FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_ColCAMTA1_F1a_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_ColCAMTA1_F1a_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562789
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC32340.5746380970488052TruSeq Adapter, Index 7 (97% over 38bp)
GGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC29180.518489167343356TruSeq Adapter, Index 7 (97% over 38bp)
CGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC9160.16276082155123858TruSeq Adapter, Index 7 (97% over 38bp)
GGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC7910.14055001075003243TruSeq Adapter, Index 7 (97% over 38bp)
TGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC7800.1385954593995263TruSeq Adapter, Index 7 (97% over 38bp)
TGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC7410.13166568642954998TruSeq Adapter, Index 7 (97% over 38bp)
GGCGACGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC7130.12669046481007978TruSeq Adapter, Index 7 (97% over 38bp)
CGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC6930.12313673508188683TruSeq Adapter, Index 7 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCCG650.070.0113751
CACGCTA3750.070.0113754
CGGGGCG2000.070.0113751
CTGCCCG650.070.0113751
GGACCGC3000.070.0113752
AGGTGCG500.070.0113751
CTGTCCG550.070.011371
TGGCCCG353.7034624E-970.011371
GGCGCTA37050.069.727934
CGGGCGC5850.069.412982
GGGCGCT25700.069.330333
AGGCGCT12500.069.1712343
CGCTAGA60900.069.149166
CGCTAGG3850.069.1021356
CCGCTCT11150.069.069525
GCGCTAG28750.069.03735
CCCGCTG3400.068.98184
CGCTATC16100.068.924246
ACGCTAT12700.068.908835
GCGCTAT31150.068.8875965
Produced by FastQC (version 0.11.5)