FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_ColCAMTA1_Col_UB_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_ColCAMTA1_Col_UB_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8906104
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC13000.023.69210850
TATGCCG15450.022.42712248
ATGCCGT15350.022.34521549
CGTATGC15950.021.50464246
GCCGTCT12250.021.4283951
GTATGCC17500.020.7999447
TCGTATG22500.015.24440245
TCGTATC35400.015.0282938
CTCGTAT23950.014.467644
CGTATCT32300.013.9782139
TATCTCG31450.012.68676941
ATTCGTA51950.012.32926736
CCGTCTT24850.011.54919952
TTCGTAT53950.011.28826237
TGGGGGG12800.010.9385131
TCTCGTA32500.010.66156943
CACGAAT60350.010.43907631
GTATCTC43450.010.23006340
TGCCGGC4854.572721E-710.10300850
CGAATTC65750.010.06092133
Produced by FastQC (version 0.11.5)