FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_ColCAMTA1_Col_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_ColCAMTA1_Col_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135104
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4600.029.67358448
TGCCGTC4650.029.35451750
GCCGTCT4700.028.29756251
CGTATGC4950.027.5754546
ATGCCGT5350.025.51373549
GTATGCC5250.025.33305447
GCGCGCA750.001833411323.3336229
CCGTCTT6900.019.2751552
CTCGTAT7050.017.87214344
TCGTATG8000.016.62481745
CATCTCG7350.015.237926541
AGGGGGG2352.769315E-614.8958941
GTCACGA10500.014.33317529
TCACGAG11650.013.21873930
AGTCACG11400.013.20160928
GTGGCCG1600.00910007713.12485524
TCTCGTA8900.012.97738543
CTGCTTG7850.012.92979459
CACGAGA12600.012.77763731
ATCTCGT9400.012.65943542
Produced by FastQC (version 0.11.5)