FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_ColCAMTA1_C24_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_ColCAMTA1_C24_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences587028
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT13290.22639465238455406TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGCC150.002224564369.9983856
GTGCGTG200.00695098752.49878329
GCCGTCT2700.051.8506651
TGCCGTC3000.051.33214650
ATGCCGT3150.047.77667249
GTATGCC3200.047.03016347
TATGCCG3250.046.3066248
CGTATGC3350.044.92433546
GACGGGC350.001254883840.00248323
CCGTCTT3500.039.99907752
TCGTATG4150.037.9509345
CTCGTAT3650.036.43751544
CTGCTTG4150.032.0474559
GTCACGA5050.031.88045129
GGCCCAC450.00430724931.11039227
AGTCACG5200.030.96082328
CACGAGA5600.030.00186231
CTTCTGC4200.029.99930856
TCACGAG5400.029.81666630
CGAGATT5650.029.73635933
Produced by FastQC (version 0.11.5)