FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_C24SPL9_F1b_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_C24SPL9_F1b_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603931
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGCG200.00695077352.49938222
GGGCGCT200.00695077352.49938258
CGCGATA350.001255368339.99952747
ATGCCGT1450.036.2064749
GTATGCC1700.032.9407947
CGTATGC1500.032.6662846
TATGCCG1750.031.99962248
TGCCGTC1551.8189894E-1229.3544950
CCGACTG500.00720811827.99966846
AGCCTGC700.001225964924.99970625
GCCGTCT1202.166329E-623.3330651
TTCGCAA952.7795119E-422.10500136
CTACCCG800.002665878721.87474370
GTAGGCC850.003788971320.58799419
GCCTGCA850.003788971320.58799426
CTCGTAT2750.020.36339644
CTGCTTG1558.736115E-720.3223459
CGTCTGC900.005274384319.44421456
TGTCGGG900.005274384319.44421463
ACGTATG950.007207231618.42083563
Produced by FastQC (version 0.11.5)