FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_C24SPL9_F1a_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_C24SPL9_F1a_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279571
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT5790.2071030257072443TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAC200.00694740452.4983924
CAAGCCG200.00694740452.4983955
AGGTCCT404.5769935E-543.7643133
TGCCGTC1250.041.99871450
GCCGTCG350.001254328339.99877551
GGTCCTA459.166352E-538.9016154
AGTGTCC459.185698E-538.88769514
GTATGCC1350.036.2951847
GCCGTCT1350.036.2951851
ATGCCGT1450.036.20578849
GAACGGT400.002416676434.99892829
TATGCCG1550.033.86992648
CGTATGC1450.033.7920746
GAGGTCC450.004296054631.1212922
CCGTCAC450.00430358531.11015759
CCCACAG450.00430358531.11015755
AACTCGC450.00430358531.11015743
GCGTCTT450.00430358531.11015752
CATTCAG3450.028.40492633
GCCGATA500.007189689728.0091615
Produced by FastQC (version 0.11.5)