FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_C24SPL9_Col_B_gnew-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_C24SPL9_Col_B_gnew-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3034049
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC5950.033.5292738
TGCGCAT7750.030.70954737
ATGCGCA7850.030.31834236
AATGCGC8450.029.4081635
TAATGCG9750.025.128134
CGCATCG5750.021.9129539
CATCGCG3850.018.18174241
TCGCGTA2501.7078492E-816.79992943
CATCGGG3950.015.94929941
GCATCGG3755.4569682E-1114.9332740
CTAATGC18150.014.65558533
ATCGCGT3403.263267E-914.41170542
GCATCGC5150.013.59217640
CGCGTAT2701.166463E-512.96290944
GTCACTA19100.012.8271729
ATCGGGG2203.9325745E-412.7272242
CGTATGC3851.8675564E-812.72721946
GTATGCC4458.221832E-1012.58421647
CAGTCAC20250.012.27155327
CCAGTCA20600.012.23295926
Produced by FastQC (version 0.11.5)