FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_C24SPL9_Col_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_C24SPL9_Col_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638964
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGTA401.3460522E-1070.0050051
CGGTAAT9000.064.560183
GCGGTAA4950.063.640922
GGGTAAT17900.063.3564873
GGGGTAA6950.062.9541442
GGCGGTA2900.062.763111
GGATAAT24500.062.575913
GAGTAAT22200.061.6485563
GCGTAAT6650.061.583353
GGGCTAA3600.061.2543832
GGGGCTA1600.061.254381
GGTAATG9950.061.210414
GGGAGTA4750.061.162271
GGCTAAT10150.061.0388533
GTAATAG13950.060.9721075
GGTAATC12600.060.8376854
GGGATAA11050.060.818832
GGTAATA26000.060.581264
GTAATCG4750.060.4253775
AGTAATC13500.060.411734
Produced by FastQC (version 0.11.5)