FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_C24SPL9_C24_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_C24SPL9_C24_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182560
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA7450.40808501314636286TruSeq Adapter, Index 13 (97% over 38bp)
GGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA6240.3418054338299737TruSeq Adapter, Index 13 (97% over 38bp)
CGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA2430.13310692375109553TruSeq Adapter, Index 13 (97% over 38bp)
GGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA2030.11119631901840492TruSeq Adapter, Index 13 (97% over 38bp)
CGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGA1960.10736196319018405TruSeq Adapter, Index 13 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCGC401.3460522E-1070.000012
CAGCGCG207.9107915E-570.000011
CTGTCAG207.9107915E-570.000018
TAGACGC207.9107915E-570.000012
ACACCGC401.3460522E-1070.000012
TGGCGCG207.9107915E-570.000011
GCTGCGA207.9107915E-570.000017
CGCGAGT207.9107915E-570.000013
CCGCTGG252.8314244E-670.000015
GGCCCCG207.9107915E-570.000011
GGGACGC800.070.000012
GAGCGTG207.9107915E-570.0000169
AGACCGC207.9107915E-570.000012
GGTATCG401.3460522E-1070.000011
ACGCTGT650.070.000015
GGGCACG207.9107915E-570.000011
CGCACCG207.9107915E-570.000011
CATCCGC207.9107915E-570.000012
GCGCTGA1600.070.000015
ACGAGCG252.8314244E-670.000011
Produced by FastQC (version 0.11.5)