FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_C24CAMTA1_F1a_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_C24CAMTA1_F1a_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649231
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC37220.5732936350852008TruSeq Adapter, Index 7 (97% over 38bp)
GGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC31720.4885780253869578TruSeq Adapter, Index 7 (97% over 38bp)
CGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC9150.14093596886162243TruSeq Adapter, Index 7 (97% over 38bp)
GGCGTCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC8850.13631511742353647TruSeq Adapter, Index 7 (97% over 38bp)
GGCGACGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC7950.12245256310927852TruSeq Adapter, Index 7 (97% over 38bp)
TGCGCCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC7640.11767768328992301TruSeq Adapter, Index 7 (97% over 38bp)
CGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC7560.11644545623976674TruSeq Adapter, Index 7 (97% over 38bp)
TGCGGCGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTC7330.11290280347056747TruSeq Adapter, Index 7 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCGC3050.070.013482
CGCACCG353.7052814E-970.013481
GCGCTAT34250.069.497085
CCGCTAG31500.069.341345
GCGCGCT15150.069.314943
TGCGCTA14750.069.2961354
GCCGCTA37350.069.258344
GGCGCTA45750.069.242974
GGCCGCT18300.069.2429663
AGGCGCT18150.069.236653
GCGCTAG32250.069.139785
CGCGCTA10850.069.040244
CGCTAGT18000.069.035756
CCGCTAA17750.069.022065
CGCTAGA63200.069.011136
GACGCTA16100.068.921014
GTGGCGC6150.068.8750462
GGGCCGC9150.068.8657152
GTTCGCT15300.068.864173
GGCGCTC18200.068.85414
Produced by FastQC (version 0.11.5)