FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_C24CAMTA1_Col_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_C24CAMTA1_Col_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2170536
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3250.022.61561650
TATGCCG3400.021.61786848
GCCGTCT3450.018.26063551
GTATGCC4050.018.14833347
ATGCCGT4750.016.9475449
TGACCGC1350.002947984715.55535742
GGCGTCC1400.00375509715.00015449
CGTATGC6400.012.57825346
TGACGCT2701.6735253E-411.6665179
AGCTGGG2550.001357845310.98025110
AGGGGGG2903.243448E-410.8634311
CATCGGC2300.00789325410.65203856
CTCGTAT7951.0913936E-119.68563344
GGTACAG9750.09.3345041
CACGAGA8657.2759576E-129.30623931
GTCACGA7951.1459633E-109.24516529
TCACGAG8504.5474735E-119.05870830
TCGTATG8951.4551915E-118.99450545
GCGGCGA3150.00780394448.88877512
AGTCACG8504.292815E-108.64694928
Produced by FastQC (version 0.11.5)