FastQCFastQC Report
Thu 4 Apr 2019
HJTFLAFXY_n01_C24CAMTA1_C24_B_g-b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJTFLAFXY_n01_C24CAMTA1_C24_B_g-b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5525508
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT75320.1363132584370523TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC15900.044.685650
TATGCCG17050.042.90328648
GCCGTCT15450.041.90906551
ATGCCGT18900.038.33338549
GTATGCC19250.038.00005347
CGTATGC22900.032.7074746
CTCGTAT23450.028.95526544
CATCTCG22100.028.0314641
TCGTATG26650.027.71111145
GTCACGA29700.026.5147129
AGTCACG30550.025.43329228
CACGAGA30750.025.26809931
CTGCTTG24600.023.75976659
TCACGAG33100.023.5798830
CCAGTCA35300.022.80435826
TCTCGTA28750.022.52156643
CCGTCTT29450.022.2239452
CAGTCAC36500.021.67087227
TCCAGTC38050.020.97224425
ATCTCGT31450.020.14293142
Produced by FastQC (version 0.11.5)