FastQCFastQC Report
Mon 20 Apr 2015
HJT3VADXX l01n02 long dna2rnh201rad27 - pnk.34200000008975.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJT3VADXX l01n02 long dna2rnh201rad27 - pnk.34200000008975.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57895512
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN885050.1528702259339204No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG610290.10541231589764677Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG317950.036.98931518
AGCACAC381150.034.2025717
TAGATCT4002050.034.1026345
AGTGTAG4055050.033.6042341
GTGTAGA4066650.033.50214842
GAGTGTA4073800.033.44357340
GAGCACA423100.033.36879316
AGAGCAC431800.033.11844615
AGAGTGT4157100.032.77103839
TGTAGAT4211750.032.362343
AAGAGTG4217800.032.34643638
GATCGAG187500.032.1474768
CACACGT198100.032.0292919
GGGAAAG4370100.031.40355734
GTAGATC4355600.031.35624744
AAAGAGT4376150.031.11737837
GAAAGAG4456900.030.79072436
GTAGGGA4470700.030.63599231
CGTGTAG4464500.030.63136928
GGTCGCC93500.030.628244
Produced by FastQC (version 0.11.2)