HJT3VADXX l01n02 long dna2rad27rnh201-frb.34200000008982.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HJT3VADXX l01n02 long dna2rad27rnh201-frb.34200000008982.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2187231
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	73212	3.347245901324551	Illumina Single End PCR Primer 1 (100% over 51bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC	12483	0.5707216110232527	Illumina Single End PCR Primer 1 (100% over 40bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG	9551	0.4366708408942631	Illumina Single End PCR Primer 1 (100% over 41bp)
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT	8332	0.3809382730950686	Illumina Single End PCR Primer 1 (100% over 39bp)
ACACTCTTTCCCTAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAT	7551	0.3452310249809005	Illumina Single End PCR Primer 1 (100% over 37bp)
GTGACCGGAGTTCAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCGTCG	5709	0.2610149545246936	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ACACTCTTTCCCTACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAG	4552	0.20811702101881327	Illumina Single End PCR Primer 1 (100% over 35bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	3258	0.14895546012286767	No Hit
GTGAATGGAGTTCAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCGTCG	3182	0.1454807471181599	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GGGACTGGAGTTCAGACGTGTGCTCTTCCGATCTAGATCGGAAGAGCGTCG	2754	0.1259126265127003	Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
ACACTCTTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG	2499	0.11425404998374658	Illumina Single End PCR Primer 1 (100% over 42bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GTCGCCG	7645	43.902523	45
GGTCGCC	7635	43.900085	44
GTGGTCG	7890	42.938427	42
TGGTCGC	7835	42.86364	43
GGTGGTC	8035	42.170326	41
CGGTGGT	8235	41.338356	40
ACACTCT	5250	40.901848	1
CACTCTT	5420	40.498512	2
ACGTGTG	4435	40.083088	16
GGAGTTC	4805	40.04886	7
CCGATCT	4540	39.749004	28
CGTGTGC	4600	39.721527	17
CTCGGTG	8685	39.717335	38
AGTTCAG	4815	39.70917	9
TCCGATC	4645	39.577114	27
TGTGCTC	4590	39.563847	19
TCAGACG	4720	39.514866	12
CAGACGT	4540	39.350704	13
CGATCTA	4340	39.341434	29
GTGTGCT	4660	39.210987	18