FastQCFastQC Report
Mon 20 Apr 2015
HJT3VADXX l01n02 long - dna2rnh201 - pnk.34200000008968.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJT3VADXX l01n02 long - dna2rnh201 - pnk.34200000008968.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977407
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG42550.4353355357594124Illumina Single End PCR Primer 1 (100% over 51bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC24840.2541418262811705Illumina Single End PCR Primer 1 (100% over 40bp)
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT15480.15837823956652652Illumina Single End PCR Primer 1 (100% over 39bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15260.15612738603263532No Hit
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG13150.13453965441213334Illumina Single End PCR Primer 1 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAG12900.042.23328
GGTCGCC4500.041.49578544
GTCGCCG4750.039.311845
ATCGAGA15650.039.2351469
GTGGTCG4900.038.5655342
TCGAGAT18900.038.44452710
AGCACAC9000.037.9941917
GCACACG7300.037.90905418
AGATCAG13250.037.7155847
AGATCGA9900.037.517387
ACACGAG3700.037.0927320
GAGCACA10750.036.41303616
CACACGA3900.036.3443319
AGAGCAC11050.036.03521315
CAGATCA8400.035.641516
ACACTCT9750.035.3041041
CCGGGCA458.7050284E-734.996451
GATCTAG7050.034.48768
GATCAGA15000.034.2193648
CACACGT3750.034.19828419