Basic Statistics
Measure | Value |
---|---|
Filename | HJT3VADXX l01n02 long - dna2rnh201 - pnk.34200000008968.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 977407 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 4255 | 0.4353355357594124 | Illumina Single End PCR Primer 1 (100% over 51bp) |
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC | 2484 | 0.2541418262811705 | Illumina Single End PCR Primer 1 (100% over 40bp) |
ACACTCTTTCCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT | 1548 | 0.15837823956652652 | Illumina Single End PCR Primer 1 (100% over 39bp) |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | 1526 | 0.15612738603263532 | No Hit |
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG | 1315 | 0.13453965441213334 | Illumina Single End PCR Primer 1 (100% over 41bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGAG | 1290 | 0.0 | 42.2332 | 8 |
GGTCGCC | 450 | 0.0 | 41.495785 | 44 |
GTCGCCG | 475 | 0.0 | 39.3118 | 45 |
ATCGAGA | 1565 | 0.0 | 39.235146 | 9 |
GTGGTCG | 490 | 0.0 | 38.56553 | 42 |
TCGAGAT | 1890 | 0.0 | 38.444527 | 10 |
AGCACAC | 900 | 0.0 | 37.99419 | 17 |
GCACACG | 730 | 0.0 | 37.909054 | 18 |
AGATCAG | 1325 | 0.0 | 37.715584 | 7 |
AGATCGA | 990 | 0.0 | 37.51738 | 7 |
ACACGAG | 370 | 0.0 | 37.09273 | 20 |
GAGCACA | 1075 | 0.0 | 36.413036 | 16 |
CACACGA | 390 | 0.0 | 36.34433 | 19 |
AGAGCAC | 1105 | 0.0 | 36.035213 | 15 |
CAGATCA | 840 | 0.0 | 35.64151 | 6 |
ACACTCT | 975 | 0.0 | 35.304104 | 1 |
CCGGGCA | 45 | 8.7050284E-7 | 34.99645 | 1 |
GATCTAG | 705 | 0.0 | 34.4876 | 8 |
GATCAGA | 1500 | 0.0 | 34.219364 | 8 |
CACACGT | 375 | 0.0 | 34.198284 | 19 |