FastQCFastQC Report
Mon 20 Apr 2015
HJT3VADXX l01n01 long dna2rnh201rad27 - pnk.34100000008978.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJT3VADXX l01n01 long dna2rnh201rad27 - pnk.34100000008978.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57895512
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACAGT4043400.033.62685845
CACACAG4103200.033.44164744
GTCACAC4114650.033.33114642
TCACACA4200600.032.6486143
AGTCACA4235300.032.44964641
CAGTCAC4253300.032.32395640
CCAGTCA4301650.031.97580739
TCCAGTC4308950.031.94356238
CTCCAGT4387600.031.42288237
ACTCCAG4410500.031.3270936
GAACTCC4436450.031.18881234
GAGATCG1572800.030.04328712
AACTCCA4611800.030.01619735
TGAACTC4650050.029.8374433
CTGAACT4766150.029.13426632
AAGATCG1380600.029.10843512
AGATCGG4805400.029.0631813
GATCGGA4807500.029.0507114
ACGTCTG4802550.029.00358628
ACACGTC4813250.028.94838326
Produced by FastQC (version 0.11.2)