FastQCFastQC Report
Mon 20 Apr 2015
HJT3VADXX l01n01 long dna2rad27rnh201-frb.34100000008985.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJT3VADXX l01n01 long dna2rad27rnh201-frb.34100000008985.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2187231
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC689753.1535306513120926TruSeq Adapter, Index 2 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACAC157470.7199513905938605TruSeq Adapter, Index 14 (97% over 38bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGT123180.5631778262104004TruSeq Adapter, Index 2 (100% over 39bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTA94730.43310468807364194TruSeq Adapter, Index 2 (100% over 40bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATG83000.3794752360404548TruSeq Adapter, Index 2 (100% over 38bp)
TAGGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA73450.33581272394182415TruSeq Adapter, Index 2 (100% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCGGTCACAGATCGGAAGAGCACAC72820.33293236974055324TruSeq Adapter, Index 13 (97% over 35bp)
TGTAGGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC45130.20633394460850268Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCATTCACAGATCGGAAGAGCACAC37170.1699408978749844TruSeq Adapter, Index 13 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATATCGTATGC27430.12540970752517683TruSeq Adapter, Index 2 (98% over 50bp)
AAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT24520.11210521430978254TruSeq Adapter, Index 2 (100% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCACTCACAGATCGGAAGAGCACAC22190.1014524757558758TruSeq Adapter, Index 13 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG78000.041.88395340
CGTATGC83200.041.2393945
ATCTCGT79950.040.94682341
TCTCGTA79800.040.88281642
CTCGTAT80100.040.84205643
TCGTATG84900.040.6256444
GTATCTC86350.037.78168539
TCCGGTC9650.037.0711126
ATGTATC88300.036.97111537
TGTATCT89450.036.62072438
CCGGTCA9950.036.40563227
CGGTCAC10150.035.465828
GTCACAG36050.035.32457430
TCACAGA49050.034.6316931
CACAGAT53850.034.26051732
ACTCCGG10850.034.007924
GATGTAT101450.033.70834736
CTCCGGT10800.033.33204325
AGTCACA23500.032.83915729
ACAGATC57950.032.72953833
Produced by FastQC (version 0.11.2)