FastQCFastQC Report
Mon 20 Apr 2015
HJT3VADXX l01n01 long - dna2rnh201 - pnk.3410000000896b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJT3VADXX l01n01 long - dna2rnh201 - pnk.3410000000896b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977407
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC37010.37865495131506116TruSeq Adapter, Index 4 (100% over 50bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCA24680.25250484189288597TruSeq Adapter, Index 4 (100% over 39bp)
GGGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC15550.159094420236401TruSeq Adapter, Index 4 (100% over 38bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAA12910.13208417782970655TruSeq Adapter, Index 4 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC6600.031.7042245
CCGTGAG501.981376E-631.4996768
ACTGACC194950.031.14997545
CACTGAC198950.030.60285444
GTCACTG202750.030.06257642
TCACTGA202500.030.03302843
CTCGTAT6100.029.87674143
AGTCACT205300.029.72205241
TCGTATG7050.029.68054844
CAGTCAC207600.029.38192440
CCAGTCA210950.028.89399139
TCCAGTC211450.028.88803338
CTCCAGT213700.028.59440837
AAGATCG62550.028.5234312
ACTCCAG216300.028.3651136
GAACTCC218800.028.1144334
AACTCCA220600.027.81363135
CGAGATC16200.027.4983111
TGAACTC224500.027.440733
TCAGATC14000.027.15904611
Produced by FastQC (version 0.11.2)