FastQCFastQC Report
Mon 2 Apr 2018
HJT2TBGX5_n02_2b_t3d14a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJT2TBGX5_n02_2b_t3d14a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35737701
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA2668960.7468191644448533No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC1376870.38527100554117905No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC1106640.30965618073753537No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC885530.24778594459671593No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT808750.22630163031472No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTC807160.22585672200906262No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC781720.21873818911854456No Hit
TCCAGCATTAGAGGTAACAACTAGAGTACAATTTTTTCCAATAGAATCAC726340.20324194888753475No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG661000.18495873587391645No Hit
GTGCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGA656850.18379749721449623No Hit
GCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCC638100.17855093700627245No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC580100.1623215774288335No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT576390.16128345804896627No Hit
GTGGTATTTCACTGTTGGTATTTGTATAATAAATACAAAAAAAACCTCCC535320.14979139256887286No Hit
ATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCC534280.1495003833626567No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT531510.14872529153456177No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA523400.1464559793591647No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG520080.14552698843162853No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT513260.14361863959855728No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG502040.14047909796995614No Hit
GGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCC481360.13469249183096585No Hit
GGCTGCTCTACTGCTTACAACACCTTGACCAGTAATCAAGTCGTCTGCAA459350.1285337296878722No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA457570.12803565623877147No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG457120.1279097387937741No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG452550.1266309771856897No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT451470.126328775317696No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT446530.12494648158816932No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT444560.12439524299562527No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT441820.12362854566386348No Hit
GGCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGC422290.11816372855097759No Hit
GCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCT417700.1168793706120044No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG413440.11568735213269593No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC412310.11537115943748033No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT406800.11382937027762362No Hit
GAGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCC406420.11372303999073695No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA406240.11367267301273801No Hit
GCCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAA403530.11291437017730939No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT400820.11215606734188079No Hit
GTGTAATCCATCTGATAAATCGAAAGGGAAACGGGTTAATATTCCCGTAC397830.11131941587400937No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA374770.10486684635925517No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT370320.1036216627365034No Hit
GAGCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTG368330.10306482781307057No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAG430300.086.37235
GGCCGAC88650.080.655761
CGGGGCA47300.072.382731
GGGGAGA640100.072.08744
GGAGAGA257800.070.896786
TAGAGGT189400.069.478869
CATTAGA196100.067.3971946
TCCAGCA199650.066.572371
GGGGGAG877350.063.7822153
CAGCATT209000.063.5871283
CCGGCAT68250.063.365241
GTCCAAT105050.057.1546251
AGGGGCA32700.056.4281
GAGAGAG323600.055.390877
GGGTCTG89350.055.2884372
TCCAATC110350.054.033462
GGTCTGG91300.054.013663
AGCATTA246800.053.6979644
GGAGTCT111350.052.2402153
GAGTCTA113050.051.195844
Produced by FastQC (version 0.11.2)