FastQCFastQC Report
Mon 2 Apr 2018
HJT2TBGX5_n02_2b_t1d9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJT2TBGX5_n02_2b_t1d9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39615246
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA3593760.9071658926464826No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1347750.3402099282685257No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1241390.31336167898591366No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC1102260.27824136192414406No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT992380.2505045658431605No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG867540.21899144586909797No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG864280.21816853036833345No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT813210.20527702894991487No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG707580.17861305215673784No Hit
GTGCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGA704160.17774974816513825No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC677930.17112855994886414No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT655870.165559996774979No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT650340.16416406956049193No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC623760.1574545315205161No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG606920.1532036428601251No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG606880.15319354573741634No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA590460.1490486768654674No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT585320.14775119659739083No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC583360.14725643758466123No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTC572750.14457817578616072No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT565590.14277079082129138No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT564290.14244263433325646No Hit
CTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCCGACAT551620.13924437071525445No Hit
GCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCC523550.13215871485437702No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA521670.13168415008706497No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA519210.1310631770404758No Hit
ATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCC516670.13042200974846907No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT508840.12844549797822788No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG499240.12602218852812375No Hit
GCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGAAA492260.12426024061544386No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT482790.12186974681414323No Hit
AGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCT468620.11829284109456242No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG459910.11609419262472837No Hit
GTTTTATTTGATATTTCGGCCGGCGTTAAACAGTCAACTTAGAACTGGCA455180.11490020786441664No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG452850.11431205046663094No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG451840.11405709811823458No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA451220.1139005927162487No Hit
GGCTGCTCTACTGCTTACAACACCTTGACCAGTAATCAAGTCGTCTGCAA448520.11321903693340689No Hit
CCGCGCTTGGTTGAATTTCTTCACTTTTACATTCAGAGCACTGGGCAGAA442430.11168175000099709No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT442400.1116741771589655No Hit
GCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTCTTGATTAATGAA436540.11019494868213112No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTCTGTGAAGAGTCATGA426490.10765804660155336No Hit
GCGGATATGGGTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATT425550.10742076421789731No Hit
GAGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCC421210.10632522640399607No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC419030.10577493321636826No Hit
GGGAAACTTCAGAGGGAACCAGCTACTAGATAGTTCGATTAGTCTTTCGC418970.10575978753230512No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC414520.10463648263095476No Hit
TGTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA411080.10376813007800079No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC403370.10182190967588589No Hit
GTGTAATCCATCTGATAAATCGAAAGGGAAACGGGTTAATATTCCCGTAC398110.100494138039683No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAC61900.079.523121
CCGGCAT63450.066.903231
CGCGGAT37700.060.7923771
CGAAGGA1039500.056.716788
GGTCTGG59500.056.38543
ATCGAAG1066300.055.5535286
GTCCAAT101600.054.2664151
GAAGGAT1095600.053.8920679
GACATCG1064500.052.5635643
GGGTCTG65250.051.9828832
CCTGGCC312550.050.0018965
TCCAATC111850.049.3832132
CGGGGCA48500.048.889721
TGGCCCG330500.047.1554647
GGGCACT18550.046.623051
GGAGTCT151300.046.348671
ACATCGA1211450.046.242144
ACCTGGC341400.045.7138634
TCAAAGG134100.045.6284527
CATCGAA1308950.045.299375
Produced by FastQC (version 0.11.2)