FastQCFastQC Report
Mon 2 Apr 2018
HJT2TBGX5_n02_2b_t1d9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJT2TBGX5_n02_2b_t1d9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47856772
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA2920050.6101644298115217No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1779120.3717592987675809No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1706520.3565890319556029No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1203440.25146702330863435No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC978590.20448307712856187No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG889930.18595696341575232No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG885680.1850688968324065No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC836020.1746921000020645No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG780380.16306574124974413No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA777990.16256633439463908No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT750890.15690360394553984No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT746610.1560092686568998No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTC736750.1539489541835375No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA711950.14876682447366069No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG692890.1447841070434086No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT689100.1439921606079073No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA681320.14236647636827657No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC615470.12860666824749484No Hit
GCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTCTTGATTAATGAA604770.12637083002589475No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT565740.11821524443813303No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG560020.11702001129537112No Hit
GCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCC558480.11669821775693523No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT553570.11567223965711687No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC545910.11407163023866299No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC544050.11368297051042223No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT542840.1134301327302226No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC536650.11213668987118479No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA513980.10739963823719662No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT512250.10703814289856406No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG508430.10623992775776854No Hit
GTGCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGA503290.10516588958402794No Hit
CTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCTAGAGTCTCGCTC496580.10376378916655725No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC491780.10276079631948432No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATC491030.10260407868712916Illumina Single End PCR Primer 1 (96% over 33bp)
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT488210.10201482038947382No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG488190.10201064125261103No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG485300.10140675597593586No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAGA597950.076.495326
AGAAAGG265650.070.754298
GGGGAGA1713850.069.175874
GGGAGAG1406650.065.459385
CGCGGAT48550.064.287291
GAGAAAG293550.063.9556547
GGTCTGG128250.063.770493
GGGGGAG2255050.061.5849883
CCGGCAT61000.057.7837221
GGGTCTG142950.057.079512
GGCCGAC69600.056.65061
GTCCAAT103250.056.199481
GAGAGAG827700.053.966257
CGAAGGA938850.052.024678
ATCGAAG953100.051.4201776
TCCAATC114400.051.1168982
GAAGGAT984900.049.6438839
GAGTCTA179350.049.510382
GGAGTCT183600.048.7220541
GGGAGAA570300.047.562095
Produced by FastQC (version 0.11.2)