FastQCFastQC Report
Mon 2 Apr 2018
HJT2TBGX5_n02_2b_c2d9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJT2TBGX5_n02_2b_c2d9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37882372
Sequences flagged as poor quality0
Sequence length150
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC990120.2613669492501684No Hit
GCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCC970880.2562880698178034No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC859370.22685221506192907No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT727920.1921526983579592No Hit
GGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGTTCCGA725120.19141356829503708No Hit
GTCCGTCCCCGCCGGCGGCCCGTCGCGCCCTCCCGTGGCGGCGCGGCGGC665000.17554338994400878No Hit
GTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCA653850.17260006844344383No Hit
GCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGTTCCGAGCCGT652810.17232553442007276No Hit
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT628540.1659188606246726No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG578420.15268843249836628No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC557120.14706576451970854No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA535330.14131374878003944No Hit
CCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGTTCCGAGCCGTG527270.13918611009891355No Hit
CGCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCC515780.13615303709070806No Hit
CCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCCC510880.1348595594805943No Hit
CGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGT497940.13144372268980412No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG473450.1249789743894601No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT469720.12399434755563879No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT457550.12078177153215222No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTG430040.11351981866394216No Hit
GCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAATGAAGG428010.11298394936832361No Hit
CGCCGGCCGAGGCGAGGCGCCGCGCGGGAAAAACCGCGGCCCGGGGGGCG418710.1105289816593322No Hit
CCGGGCCGCCCCTCCCACGGCGCGACCGCTCTCCCGGCCCCCTCCCCCGC410100.10825615671584661No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT384790.10157494889707538No Hit
CGGGCCGCCCCTCCCACGGCGCGACCGCTCTCCCGGCCCCCTCCCCCGCC380820.10052696805786078No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTA277050.054.9732322
AGTCTAA267200.053.670063
GGGCATA60050.049.7488174
TCTAACG337100.042.6016775
GTCTAAC348500.041.229254
GGAGTCT376750.040.784051
CTTGTTA41650.035.4727861
TCCACTC178850.032.0093772
AACGACG70850.031.8013736
CCTGGCC135650.031.2563325
GTCGGAT147450.030.8907721
GGCATAG606750.030.514325
GTTACGA48000.030.2940834
AGAAACT125800.030.2729173
GCATAGT624850.029.8261816
CCAGGCA491200.029.071232
TTCCACA569000.028.9712667
GAAACTA133050.028.7835584
TAGTTCA663450.028.7209369
ATCGAAG270100.028.7033446
Produced by FastQC (version 0.11.2)