FastQCFastQC Report
Mon 2 Apr 2018
HJT2TBGX5_n01_2b_t3d14a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJT2TBGX5_n01_2b_t3d14a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35737701
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT10866363.040587305825856TruSeq Adapter, Index 19 (97% over 38bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA2686750.7517971007704161No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC1369890.3833178860609976No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC842710.23580420016385498No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC838790.23470731930965563No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT836790.23414768622077842No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC828460.2318168144056049No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG742650.20780575672732837No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC638850.1787607994146014No Hit
GCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCC625750.1750952026824557No Hit
GTGCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGA624530.17475382649824062No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTC612800.1714715784319758No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT602910.16870419280747803No Hit
ATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCC565100.15812432926225445No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT556600.15574588863452632No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG541610.15155143863339166No Hit
GTGGTATTTCACTGTTGGTATTTGTATAATAAATACAAAAAAAACCTCCC523080.14636643806494434No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT517170.14471272228731222No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG516000.14438533693031905No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA512900.14351790564255937No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG506880.141833410045039No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA503660.1409324007719467No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG498370.13945217125186649No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT488750.13676033609436714No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT473560.13250992278434473No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT471710.13199226217713333No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT468390.13106327124959716No Hit
GGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCC461830.12922767471807994No Hit
GTGTAATCCATCTGATAAATCGAAAGGGAAACGGGTTAATATTCCCGTAC459390.12854492234964976No Hit
GAGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCC433170.12120813255446959No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT422080.11810496707664547No Hit
GGCTGCTCTACTGCTTACAACACCTTGACCAGTAATCAAGTCGTCTGCAA421290.11788391200653899No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG421220.11786432484842826No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT420440.11764606794376616No Hit
GCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCT410290.11480593001771434No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA409460.1145736822858303No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC408530.11431345289950241No Hit
GCCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAA407680.11407560883672958No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT402440.11260937014387132No Hit
GGCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGC394340.11034285613391863No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA374560.10480808488492308No Hit
GAGCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTG369110.10328308471773268No Hit
TGTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA361190.10106693768577894No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA361140.10105294685855701No Hit
AGCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGC360280.10081230463033983No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAC83300.080.130581
CGGGGCA40150.065.279881
CCGGCAT61600.064.991661
AGGGGCA30250.061.412651
GTCCAAT100650.057.5898061
TCCAATC102800.055.8211672
GGTCTGG80150.054.617833
GGGTCTG81850.053.835222
GAGTCTA118150.050.8848274
GGAGTCT118100.050.8452643
TCAAAGG123250.048.7214477
AGTCTAG104550.048.069215
CGAAGGA967550.046.9568988
CAAAGGA131100.045.6395958
ATCGAAG1001550.045.5060426
GAAGGAT1009250.045.130899
GGTCCCT24450.044.4694371
CGCGGAT32850.043.838751
GACATCG954400.043.5517353
CGCAGGC184550.043.422563
Produced by FastQC (version 0.11.2)