FastQCFastQC Report
Mon 2 Apr 2018
HJT2TBGX5_n01_2b_t2d9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJT2TBGX5_n01_2b_t2d9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33089323
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT10532233.1829693221586917TruSeq Adapter, Index 19 (97% over 38bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA2721310.8224133204538516No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1209800.3656164255763105No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1020860.3085164359512583No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC916740.27705009256309054No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT782540.23649320356297407No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG753520.2277230029759146No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG744770.225078645459141No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT744210.2249094065780675No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG589120.1780393028893338No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC552230.16689069159861628No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA548970.16590547954093832No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG531920.16075276003682518No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT529090.15989749926282867No Hit
GTGCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGA527890.15953484451767116No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT504380.15242983363545998No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT494050.149307980704229No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG490780.14831974652367472No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT474400.1433695092522745No Hit
CTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCCGACAT473100.14297663327835386No Hit
ATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCC471230.1424114963004834No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA470890.14230874412268876No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC461320.1394165725300575No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT458730.13863384270509252No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT448120.13542737033332475No Hit
GCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCC428470.1294888988813703No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA428230.12941636793233877No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC413410.12493758182964335No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT402990.12178852979252551No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT401820.12143494141599694No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG390860.11812269474355822No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTC390860.11812269474355822No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG386070.11667509788580444No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG385530.11651190325048355No Hit
AGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCT382520.11560224426471342No Hit
GTGTAATCCATCTGATAAATCGAAAGGGAAACGGGTTAATATTCCCGTAC370510.11197267469026187No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG370080.11184272340658043No Hit
GCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGAAA366630.11080009101425255No Hit
TGTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA365450.11044348051484763No Hit
GTTTTATTTGATATTTCGGCCGGCGTTAAACAGTCAACTTAGAACTGGCA363770.10993576387162711No Hit
GAGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCC360200.10885686600478348No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA358270.10827359628965512No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG354910.10725816300321406No Hit
GGCTGCTCTACTGCTTACAACACCTTGACCAGTAATCAAGTCGTCTGCAA354190.10704057015611954No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC351190.10613393329322574No Hit
CCGCGCTTGGTTGAATTTCTTCACTTTTACATTCAGAGCACTGGGCAGAA346700.10477700012176132No Hit
GCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTCTTGATTAATGAA345610.10444758872824324No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC344900.10423301800402504No Hit
CTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCTAGAGTCTCGCTC334290.10102654563225726No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC331540.10019546184127127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAC52950.066.7691
GGTCTGG60700.063.5787163
CCGGCAT50450.062.370551
GGGTCTG62350.061.43422
GTCCAAT81600.057.7094421
CGAAGGA781400.056.58568
ATCGAAG807050.054.89396
CGCGGAT30450.054.3875921
GAAGGAT822850.053.8140149
GACATCG809800.052.1376953
TCCAATC93450.049.8494072
CCTGGCC251150.048.3061455
TGGCCCG260800.046.767927
ACATCGA895100.046.6302154
CATCGAA981550.045.1713335
GGAGTCT120200.044.5684741
GAGTCTA120700.044.3217122
ACCTGGC276000.043.982934
CTGGCCC279400.043.550986
TCAAAGG118200.043.432457
Produced by FastQC (version 0.11.2)