FastQCFastQC Report
Mon 2 Apr 2018
HJT2TBGX5_n01_2b_c2d9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJT2TBGX5_n01_2b_c2d9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37882372
Sequences flagged as poor quality0
Sequence length150
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGC1036650.27364970704579955No Hit
GCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCC946370.2498180420170099No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACGCGTGCGCTCGTGCTC914210.24132860529430417No Hit
GGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGTTCCGA776470.20496868569898422No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT733340.19358344297975852No Hit
GTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCA726630.19181217057897007No Hit
GCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGTTCCGAGCCGT705500.18623437835413262No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT689130.1819131072362628TruSeq Adapter, Index 13 (97% over 38bp)
CTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAAT688840.1818365544797459No Hit
GTCCGTCCCCGCCGGCGGCCCGTCGCGCCCTCCCGTGGCGGCGCGGCGGC659200.17401233481367007No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG602900.15915054104848556No Hit
CCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGTTCCGAGCCGTG574540.15166420941117414No Hit
CCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCCCC556890.14700505026453994No Hit
CGCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGCC555050.1465193362231911No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA551620.14561390189611148No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC543910.14357865447285084No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT503420.13289030581295172No Hit
CGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTTGCTCGT501530.13239139302047928No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG485160.12807012190260947No Hit
CGCCGGCCGAGGCGAGGCGCCGCGCGGGAAAAACCGCGGCCCGGGGGGCG476830.1258712099654161No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT464530.12262431718900813No Hit
AAGGAGTCTAACGCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTG462440.12207260939204123No Hit
GCGTGCGCGAGTCAGGGGCTCGTCCGAAAGCCGCCGTGGCGCAATGAAGG439800.11609621488327077No Hit
CCGGGCCGCCCCTCCCACGGCGCGACCGCTCTCCCGGCCCCCTCCCCCGC398370.10515972970224778No Hit
GCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATT392870.10370786707865072No Hit
GCGCCAGGTTCCACACGAACGTGCGTTGACGTGACGGGCGAGAGGGCGGC379120.10007821051965805No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTA302450.057.3003582
AGTCTAA295650.055.3794443
GGGCATA59350.054.7133064
TCTAACG372050.043.9106985
GTCTAAC384800.042.4370424
GGAGTCT411600.042.423361
AACGACG63450.035.9722066
CTTGTTA40650.033.8330761
CCTGGCC143250.033.5249675
AAACGAC72550.032.5516935
TCCACTC181000.031.9824942
AGAAACT126800.031.5712323
GTCTATT25100.031.5542896
GAAACTA131050.030.602394
GGCATAG615350.030.3518315
GTCGGAT159450.030.121041
TTACGAC45950.030.0851975
GTTACGA45750.030.059344
GCATAGT637950.029.4911826
CGCTAGG21650.028.9333364
Produced by FastQC (version 0.11.2)