FastQCFastQC Report
Mon 2 Apr 2018
HJT2TBGX5_n01_2b_c1d9a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJT2TBGX5_n01_2b_c1d9a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35059756
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA2915390.8315488561871337No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT2200420.6276198841771745TruSeq Adapter, Index 13 (97% over 38bp)
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1211860.3456555715904013No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1116120.3183479086391816No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC882610.25174447876933315No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG863160.24619680753054868No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG858940.24499314826948596No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT853390.24341013668206934No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT815910.23271981698902866No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT666980.19024091325678366No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA665820.1899100495736479No Hit
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT634060.18085123011124207No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC632900.18052036642810634No Hit
CTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCCGACAT613220.17490709290732087No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT605030.17257108121345738No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG584430.1666953985646677No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT568240.1620775683664199No Hit
GCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGAAA547460.15615054480128154No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG543300.15496399917900172No Hit
GTGCGAGTCGTTAAATCACCGAGTGATTTAACTTAAATCAAAAATTTCGA538830.15368903308967696No Hit
GCCAGTTCTAAGTTGACTGTTTAACGCCGGCCGAAATATCAAATAAAACA526960.1503033848837967No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG513860.14656690708286732No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT512230.146101986562599No Hit
ATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGCCTCCC509880.14543170237693612No Hit
GGGGCATTAGCTGTAATAGTATGAATATGTTCATAATTACATAATAGTGC495280.14126738360643468No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG491460.14017781527059114No Hit
GTGTAATCCATCTGATAAATCGAAAGGGAAACGGGTTAATATTCCCGTAC484270.13812703088977574No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC474710.13540025777703643No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT471140.13438199626945493No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG470590.1342251212472785No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT465050.13264496193299236No Hit
GCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCC459570.13108191625748908No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA456500.13020626840643157No Hit
GTCAAAACGTAACGCAGGTGTCCTAAGGCTAGCTCAGAGAGGACGGAAAC449200.12812410902118088No Hit
AGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCT428590.1222455740992607No Hit
GCGGATATGGGTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATT425290.12130432396620217No Hit
GGAGTCTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGA424350.12103621029193701No Hit
GACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTG421400.12019478971844527No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTCTGTGAAGAGTCATGA410530.11709436882561305No Hit
TGTTGCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACA402370.11476691395114103No Hit
GTTTGACTGGGGCGGTACATCTGTCAAAACGTAACGCAGGTGTCCTAAGG400120.11412515249678293No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG389700.11115308389482231No Hit
GCGATAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTG382260.10903099268574487No Hit
CCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAATCACCGAGTGATTTA381930.10893686767243901No Hit
GAGACAGGTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCC376850.10748791292215497No Hit
GTTTTATTTGATATTTCGGCCGGCGTTAAACAGTCAACTTAGAACTGGCA375180.10701158330936474No Hit
GCAGAATTCGCCAAGCGTTGGATTGTTCACCCACCAATAGGGAACGTGAG365200.10416501472514526No Hit
CTTGACTCTAGTCCGACTCTGTGAAGAGTCATGAGAGGTGTAGCATAAGT361260.10304121911173597No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA357420.10194594622963149No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT355600.10142683251988405No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTC355280.10133555977970869No Hit
GGGAAACTTCAGAGGGAACCAGCTACTAGATAGTTCGATTAGTCTTTCGC354670.10116157111874936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAC45950.073.650911
CCGGCAT54200.066.691461
GGTCTGG56300.058.061123
CGAAGGA884650.053.9131668
GGGTCTG61350.053.3989752
GGGCACT14600.052.771171
ATCGAAG911750.052.5154276
GAAGGAT937200.050.8749669
CGCGGAT24700.050.4330141
GACATCG911250.049.651983
CCTGGCC257500.049.6038675
GGAGTCT150300.048.09951
TGGCCCG266600.047.9653437
GAGTCTA154200.046.6462972
CTGGCCC287500.044.5031366
AGTCTAG142200.043.9499133
CCGGTCA60150.043.8110243
CATCGAA1100350.043.5075535
ACCTGGC292600.043.5057334
GCCCGGA296900.043.4585279
Produced by FastQC (version 0.11.2)