FastQCFastQC Report
Mon 2 Apr 2018
HJT2TBGX5_n01_2a_t6d14b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJT2TBGX5_n01_2a_t6d14b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40234508
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT7549571.8763917779235675TruSeq Adapter, Index 3 (97% over 36bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA1952950.48539179353205963No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC1572940.39094301836622436No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC1501210.37311503846399713No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1303190.3238985797962286No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG1163520.28918459746046854No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG877700.21814607500606195No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC854220.21231028847177652No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT716350.17804368329792924No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC701880.17444726800188534No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA692680.1721606736187752No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT648210.16110797229085044No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT646100.1605835468399415No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC615960.15309246480657848No Hit
GCGGTATCTGATCGCCTTCGAACCTCTGACTTTCGTTCTTGATTAATGAA612950.1523443507747131No Hit
GTCTGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTA598930.1488597797691474No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTGCAACTTTCCCTCACGGTA583630.14505707389288816No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG575150.14294943037454316No Hit
GCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA571930.14214912234045463No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA569810.14162221146086837No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT568310.14124939715927431No Hit
GGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTC543090.13498114603513978No Hit
CGCAAATGAACTCGGAAACATTGAGGAAAGCCCGAGCCAGAGTTCTCTTT530100.13175257418333536No Hit
GGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGA530080.1317476033259808No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA525850.1306962669954856No Hit
CCCGCATTGAGCACTCTAATTTTTTCAAGGTAAACTTGCTAGCCACCATA510370.12684882340303502No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC503100.12504191675464255No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC498860.12398809499547007No Hit
GGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCA498750.12396075528001982No Hit
GCGGATATGGGTACGATCTGGCACGAAATTCAAATAGCTTCCCTCGGATT498280.12384394013218702No Hit
TGGAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCC480500.11942484794395895No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT474540.11794353245229196No Hit
CTGGACGATAAAATTATCCAGTAACTATTTAGTAGGCTAGAGTCTCGCTC473670.1177273001573674No Hit
GGAATAATGAAATAGGATCTCGGTTCTATTTTGTTGGTTTTCTGATCTGA466190.11586819950675177No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGC465060.1155873460662176No Hit
GTGGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATG462650.11498835775498983No Hit
CTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTCAG459550.1142178748650288No Hit
GTAACTTTCACTTGAAATAATAGTGACATGTATCGATTATAATTTTGCTT429840.10683366626478942No Hit
GTTGTGGCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGG425370.10572267964603917No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT419980.10438303358897791No Hit
GAGGTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGG405390.10075679314880649No Hit
GTATTGACAAGGCTGACTAATACCGACGACTCGCAAGCGCTATCCGGCCA404920.1006399780009737No Hit
GGAAAACTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTC403910.10038894970456702No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGG135100.075.251583
GGGTCTG148150.068.574262
GTTGTGG97850.068.363211
GGCCGAC45850.060.4629021
CCGGCAT40850.059.578861
CGAAGGA599600.054.7814068
GTCCAAT69200.054.4205781
TGTGGCG123200.053.8835873
ATCGAAG620100.053.120996
TGGGTCT116800.052.0315971
GAAGGAT638450.051.5946669
CGCGGAT43750.051.3504331
TGCCGCA38850.050.0426561
GACATCG673200.047.337313
CCTGGCC416800.046.6588135
GGGCACT14700.046.534371
TCCAATC81550.045.8225362
TGGCCCG432450.045.1370437
GGAGTCT156600.044.6013031
GAGTCTA157100.044.5477522
Produced by FastQC (version 0.11.2)