FastQCFastQC Report
Mon 2 Nov 2020
HJNTYDRXX_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNTYDRXX_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493511620
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAACCAACCGGGA94124340.6302216784895186No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGG64509130.4319292139153226No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGAAGCGGCAAAAGCTC48874330.3272443906395586No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18171830.12167183540225819No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCATATCAATAAGCGGAGGAA16216190.10857759513113129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT148626500.081.64211
GCTCGAT37039200.077.524067
CTCGATG37308300.076.993588
CGGCTCG2542350.076.901165
AGCAGTG159143450.076.231542
CGCTCGA7925750.074.357696
GGCTCGA12540900.074.151366
CCGCTCG1733600.069.3778465
GCAGTGG182510450.066.491153
AGCTCGA9042750.064.0731056
TCGCTCG2445350.063.8834425
GGGCTCG4996450.063.828275
CAGTGGT195144200.062.172244
AGGCTCG3063650.061.3928835
AGTGGTA206827400.058.66555
TCGATGA49340650.058.4621169
CGCGCTC646300.057.1807144
GAGCTCG2923900.056.0537835
TGCTCGA13991250.055.8031656
GTGGTAT220350700.055.0900656
Produced by FastQC (version 0.11.5)