FastQCFastQC Report
Mon 2 Nov 2020
HJNTYDRXX_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNTYDRXX_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493511620
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTGCGG1103181647.386495191781635No Hit
TATGAGCT1068826907.156468591787722No Hit
GGCTGTTG1045576487.00079240093224No Hit
CACGGTGC1009905426.7619522103216045No Hit
ACACCTTC958445936.4173985469225885No Hit
TTTCCACT951733206.37245259598315No Hit
CCGATAGC949170546.35529397488049No Hit
ATACCCAA935832866.265989815332003No Hit
GCGATGTG872728085.843463608271089No Hit
GGTATGCA863328115.780524894744374No Hit
AGATACAA800351965.358859946466302No Hit
CTCGAAAT750800115.027079133137243No Hit
GGGGGGGG669425774.482226726833233No Hit
GTGGCAAT600405434.020092123555089No Hit
AGCATCCG564745633.7813273257291433No Hit
TAATGGGA562297803.764937563726488No Hit
CCTCATTC524633383.512750573711639ABI Solid3 Adapter A (100% over 8bp)
AGCGCCTA30322540.20302848396988032No Hit
TCAGGGTG28533580.19105027117231269No Hit
GTGCCCGT25862820.17316785255410333No Hit
GCCATTCC23106310.154711283732764No Hit
TACGTGAC22789370.15258917101696204RNA PCR Primer, Index 17 (100% over 8bp)
TTGTTGAT22588900.1512468982330382No Hit
GCTCAACC22583390.15121000531619566No Hit
GTTTATCT21929070.14682892122392727No Hit
CAAAGTGG21441720.14356580633768354ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
TTGCCGGA20625660.13810177118005953No Hit
CCAAGATG20423520.13674831669538667No Hit
AGGCCCGA19988550.13383591886616858No Hit
AGCTATCA16180200.10833661943654646No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)