FastQCFastQC Report
Wed 19 Aug 2020
HJNNJDRXX_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNNJDRXX_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856547807
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGG63668290.34293913552854716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT79589950.078.822361
AGCAGTG85384000.073.486762
CGCTCGA3003950.072.4621056
GGCTCGA4889400.070.375926
CGGCTCG1085050.067.263165
CTCGATG16124800.066.133388
GCTCGAT16635100.064.045917
GCAGTGG101084550.062.09313
CAGTGGT112836650.055.6293334
GGGCTCG2150950.055.5710565
CCGCTCG829400.052.959525
AGTGGTA126867800.049.5545275
GCGCTCG1489150.047.4522865
TGGCTCG1829750.046.0444225
TCGCTCG1320500.043.603815
GAGCTCG1433950.043.0109255
GTGGTAT148849600.042.3003966
AGGCTCG1659950.042.003915
AGCTCGA5193350.041.1756026
GTATCAA155243150.040.552399
Produced by FastQC (version 0.11.5)