FastQCFastQC Report
Wed 19 Aug 2020
HJNNJDRXX_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNNJDRXX_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856547807
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCGCGC1631779558.78932146992108No Hit
AGGTACGC1176093326.334839940914056No Hit
AAGCAGTC1171995096.312765475691302No Hit
TAAGTATG1148323326.18526124493168No Hit
AGTCCCAC1145115116.167980731131261No Hit
GGCGAGTA1143140796.157346369912251No Hit
CCCAGGAT1116779456.0153551973682085No Hit
CTTTGCGG1101506085.933087614802262No Hit
GCGAGAGT1078171685.807400573983712No Hit
CTATAGTG1076777185.799889321137207No Hit
CAAATACG1056727505.691894903086651No Hit
GCAGTTCT1046732295.6380572913520455No Hit
GTTCCTCA1033904815.56896410693937ABI Solid3 Adapter A (100% over 8bp)
ACTTCTAT957687765.158433068026025No Hit
TGCACAAA955403165.146127432849888No Hit
TACGTTCA848548344.5705709101624015No Hit
GGGGGGGG118936330.640631658132141No Hit
CCTTCTAT19571700.10541985466900504No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)