FastQCFastQC Report
Mon 2 Nov 2020
HJNMWDRXX_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNMWDRXX_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000048766
Sequences flagged as poor quality0
Sequence length91
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAGAAACCAACCGGGA41775140.4177310289286433No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTTGACCTCAAATCAGGTAGG23219900.2321876771357368No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15411300.15410548489192377No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGTGAAGCGGCAAAAGCTC13210020.13209375831578196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAT33029050.078.9775547
CTCGATG33133400.078.748398
CGGCTCG2236700.078.270955
AAGCAGT49561300.077.172941
GGCTCGA10975700.076.9038546
CGCTCGA7060200.075.561916
AGCAGTG51334800.074.469812
CCGCTCG1498650.073.2590945
GGGCTCG4087100.070.7408755
TCGCTCG2055550.068.710155
AGGCTCG2514450.067.8639455
AGCTCGA7939150.066.250556
GCAGTGG59465450.064.318953
TCGATGA41274750.063.4054459
GAGCTCG2358700.063.2592165
CGCGCTC535650.062.0570374
TGCTCGA11555950.061.5005846
TGGCTCG3453150.061.312685
CAGTGGT64114100.059.65064
AGTGGTA69591900.054.9763685
Produced by FastQC (version 0.11.5)