FastQCFastQC Report
Mon 2 Nov 2020
HJNMWDRXX_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNMWDRXX_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000048766
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCCCG700752387.007182087758308No Hit
GGGGGGGG692555926.9252214846490805No Hit
CTGACGCG691776946.917432064507942No Hit
GAGCAAGA660235336.602031345339412No Hit
TCCTTCTT644781926.447504780981851No Hit
TCTGTGAT619742416.197121891153856No Hit
TGCTCGTA613664956.136350254743477No Hit
CCAGGAGC582827535.827991092186399No Hit
ATGAATCT551111115.510842358261557No Hit
GGTCGTAC527678815.276530784699753No Hit
CGCAGTTC527645285.276195501050196No Hit
GTCGATGC514831425.148063149552449No Hit
CAGCCACT505707395.0568272987599485No Hit
AAAGAAGA471952984.719299658632847No Hit
GATCTCAG449327754.49305839151448No Hit
TGATTCTA389543983.895249844246095No Hit
ACTAGGAG324904513.248886664792905No Hit
CACGTCGG31336580.3133505191485832No Hit
TGCGGCTT28414340.28412954413865055No Hit
GCCGAGAT26546450.2654515549894694No Hit
GTATGTCA23090470.23089344025049274No Hit
ACGACATT22182070.22180988321923492No Hit
GTACGCGG20141340.20140357835309805No Hit
TAATGACC19931040.1993006809029951No Hit
AGCACACT18199760.18198872513782993No Hit
ATGCCTTA17715300.17714436137807305No Hit
CGTATCGG16787710.16786891370455428No Hit
CCTTTGTC15717160.15716393574350954No Hit
ATGAAGAC15351920.15351171384776252No Hit
TGTCAGAC15239710.15238966856542274No Hit
ACTCGGAG14518340.14517632033156272No Hit
GATTCTGG14101350.14100662367099007No Hit
CCACTACA12950860.12950228469158473No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)