FastQCFastQC Report
Fri 8 Nov 2024
HJNLYAFX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJNLYAFX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11227269
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7687716.8473553096483215No Hit
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1495911.3323899160160855Illumina Single End PCR Primer 1 (100% over 38bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1184791.0552788928456243Illumina Single End PCR Primer 1 (100% over 50bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCC1048440.9338335083981688Illumina Single End PCR Primer 1 (97% over 38bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCAGATCGGAAGAGCGTCGTGTA464990.4141612710980738Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATCACGGTGGTCGCC323120.287799285828103Illumina Single End PCR Primer 1 (97% over 38bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATAACGGTGGTCGCC302710.2696203324245638Illumina Single End PCR Primer 1 (96% over 25bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCAGATCGGAAGAGCGTCGTGTAG279710.2491344956640836Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGAAATCGGTGGTCGCC250650.22325108626149423Illumina Single End PCR Primer 1 (95% over 24bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC226680.20190128160285462Illumina Single End PCR Primer 1 (100% over 38bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCC223920.199442981191597Illumina Single End PCR Primer 1 (97% over 38bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGAAAACGGTGGTCGCC184830.16462596558432865Illumina Single End PCR Primer 1 (95% over 24bp)
GTGACTGGAGTTCAGACGTGTGCTCTTCCGACTAGATCGGAAGAGCGTCG179310.1597093647618134Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCC175570.15637818956684835Illumina Single End PCR Primer 1 (97% over 38bp)
GTGACTGGAGTTCAGACGTGTGCTCAGATCGGAAGAGCGTCGTGTAGGGA170800.15212960516043572Illumina Single End PCR Primer 1 (100% over 25bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATATAGGTGGTCGCC147550.13142109626125462Illumina Single End PCR Primer 1 (96% over 27bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGAGTAGATATCGGTGGTCGCC145490.12958627783835944Illumina Single End PCR Primer 1 (96% over 25bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGAACACGGTGGTCGCC139140.1239304055153573Illumina Single End PCR Primer 1 (96% over 26bp)
ATAACTGAAGATCGTCGTGTAGGGAAAGAGTGTAGATCTAGGTGGTCGCC137470.12244295562883548Illumina Single End PCR Primer 1 (97% over 38bp)
GTGACTGGAGTTAGATCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATC128440.11440003797895998Illumina Single End PCR Primer 1 (97% over 38bp)
ATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC125380.11167453100126129Illumina Single End PCR Primer 1 (98% over 50bp)
GTGACTGGAGTTCAGACGTGTGCTCTAGATCGGAAGAGCGTCGTGTAGGG118240.1053150147199644Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)